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DHIA now offers the PathoProof
Mastitis PCR Assay that has been used in Europe and Canada for over a year using DHIA samples to test for specific mastitis pathogens.
The test was developed by Finnzymes, a Finnish company, and was recently sold
to Thermo Fisher Scientific. It is currently in use in 15 countries around
the world. Minnesota DHIA is offering the test immediately for our
customers. The test will be run for us at Dairy Lab Services in Dubuque Iowa for now. If there is sufficient interest, the test may be added at our
labs.
Proof
Mastitis Test is a revolutionary test for identifying all the main mastitis
causing bacteria from bovine milk samples without any bacterial culturing
steps. The test may be done using the same milk sample that DHIA
collects. The Proof Mastitis Test can be used for testing selected
cows, quarters, or for Bulk Tank samples.
PathoProof
Mastitis Test Major-3 Kit identifies
1 .Mycoplasma bovis
2. Staphylococcus
aureus
3. Streptococcus
agalactiae
Cost: $22 per sample
Advantages
- Accurate
identification of all major mastitis causing pathogens
- Can
be used on treated cows
- High sensitivity even for samples
that show “no growth” in culture
- Suitable
for preserved milk samples
- Samples
can be shipped at room temperature.
- The
results are obtained in just 4 hours, with only two hours of hands-on
time.
Sensitive and
accurate results
In
addition to identifying viable mastitis bacteria, the Proof Mastitis Test
also detects growth-inhibited micro-organisms. This is a major advantage
compared to the conventional bacterial culture method, where up to 40 % of
“no growth” results are a problem for laboratories, veterinaries and dairy
producers.
The
Proof Mastitis Test simultaneously detects all of the targeted bacterial
species present in the milk sample, overcoming the common problem of ‘mixed
growth’ results of bacterial culturing.
Results
interpretation and reporting made easy
The
Proof Mastitis Test Software performs automated interpretation and reporting
of the results obtained using the assay. Unlike with identification of
bacteria from culture plates, all users of the Proof Mastitis Test can now
reach the same very high level of accuracy. The results are easy to understand:
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Meanwhile, we remind you that several of our DHIA
Field Reps have been trained and can provide sample collection services for
mastitis culturing at the University of Minnesota, the Stearns DHIA Lab, or
your local vet clinic in some cases
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What do your SCC numbers mean on the Herd Summary:
Current
SCC Evaluation
• Schematic of where the herd
currently is in regard to SCC and used to find out who and how bad the
problem is. The information is categorized by first, second, and
other lactation animals as well as an average for the entire herd.
• Linear scores of 0 and 1
are considered negligible with respect to
mastitis. Scores of 2 and
3 are
suspicious.
• Cows with a linear score above 4
(200,000) are considered likely to be infected with mastitis.
• Those above 7 (1,600,000)
probably have clinical signs of mastitis and may have a significant impact
on Bulk Tank SCC.
• Monthly SCC $ Loss – Dollars
lost due to production loss. Calculated by using current linear score times
yield loss times average milk price.
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Goals
for lowering SCC:
A. 90% of 1st lactation
cows should be in 0-3 range.
B. 80% of the whole herd should be
under a Linear Score of 4.
C. Less than 20% of the herd
should be infected.
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2009 3 Year Improvement in SCC
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3 Years Ago
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Current
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Rolling Average
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Rolling Average
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Difference
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Herd
Owner
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Town
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Breed
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Cows
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SCC
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Cows
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SCC
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SCC
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HUONDER, JOHN TONNIE
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MILACA
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XX
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72
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1,238
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91
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805
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-433
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KROSKA, JAY
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FOLEY
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XX
|
22
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727
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29
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303
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-424
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MILBRANDT BROTHERS
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ELMORE
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HO
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143
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703
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166
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282
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-421
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DEUTZ, DAN
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LAKE BENTON
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HO
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66
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727
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67
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318
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-409
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SCHMIDT, GENE
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COSMOS
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HO
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29
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547
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34
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146
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-401
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TRIPLE S JERSEY
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ROCHESTER
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JE
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37
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671
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31
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286
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-385
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HESSE, JIM
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CHASKA
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HO
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99
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486
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101
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137
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-349
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RASCHKE, LOREN
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RUTHTON
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HO
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32
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515
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32
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184
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-331
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RINDE FARMS LLC
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LONG PRAIRIE
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HO
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54
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532
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110
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202
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-330
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SCHWARTZ DAIRY
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SLAYTON
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HO
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101
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484
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88
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155
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-329
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MARXHAUSEN, EUGENE TIM
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ROLLINGSTONE
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HO
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214
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666
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268
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339
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-327
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JET-FARMS
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SPRING GROVE
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HO
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71
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684
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77
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361
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-323
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PRATT, RYAN
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ALBANY
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HO
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65
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608
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61
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299
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-309
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ROSTAD FARMS
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ZUMBROTA
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HO
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53
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613
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66
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306
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-307
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GERTNER FAMILY, BRAD
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WESTBROOK
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XX
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76
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629
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60
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326
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-303
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PLEASANT VIEW DAIRY
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LAKE CITY
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HO
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48
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821
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57
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530
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-291
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LORENZ, ANDREW
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BRICELYN
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HO
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190
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545
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90
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258
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-287
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NIX, TIMOTHY
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PLATO
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MS
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56
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583
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47
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299
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-284
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RYNDA, FRANCIS &
THERESA
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MONTGOMERY
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HO
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48
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418
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49
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138
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-280
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RUEGG, SCOTT
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PINE ISLAND
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HO
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79
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711
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79
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442
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-269
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RIOPEL DAIRY
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HUGO
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HO
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34
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847
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36
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578
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-269
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STOTERAU, CONRAD
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EAGLE BEND
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HO
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33
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566
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32
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301
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-265
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GENGLER, BRIAN & JOYCE
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ADRIAN
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HO
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71
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472
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72
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208
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-264
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KURTH, ROGER
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BUFFALO LAKE
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HO
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21
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802
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35
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543
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-259
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WEIS BROS
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PLAINVIEW
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HO
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59
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862
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66
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604
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-258
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CLOOSE, RODNEY
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SOLWAY
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HO
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64
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478
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46
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222
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-256
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KUKLOK, JOE & KAREN
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RICE
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HO
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6
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832
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11
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577
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-255
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HOFFMAN FARM 2
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MILACA
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GU
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57
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650
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40
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396
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-254
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PETERSON, MARK
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WILLMAR
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HO
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45
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754
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39
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502
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-252
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ZILKA, GREGORY P.
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LITTLE FALLS
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HO
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33
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333
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35
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86
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-247
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TOFT FARMS INC.
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RICE LAKE
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HO
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33
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528
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60
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283
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-245
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IRISH CREEK DAIRY
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SWANVILLE
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HO
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113
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489
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122
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248
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-241
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MEYER, JAMES
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GLENCOE
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HO
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55
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520
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57
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282
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-238
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HOME PLACE DAIRY LLC
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HINCKLEY
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HO
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54
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599
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53
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363
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-236
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UTHKE FAMILY
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EYOTA
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HO
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43
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513
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45
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280
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-233
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RIESGRAF, JEFF
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JORDAN
|
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XX
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67
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551
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66
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319
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-232
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FLATLAND, STEVE
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MILACA
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HO
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27
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554
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35
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322
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-232
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FLODQUIST, JAY
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NORTH BRANCH
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XX
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103
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426
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96
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195
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-231
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ASMUS, DARREL
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GREY EAGLE
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HO
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27
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558
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26
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328
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-230
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MOULZOLF PINE-GROVE FARM
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FOLEY
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HO
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136
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585
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146
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357
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-228
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WIEBE, LEROY
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OTTERTAIL
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XX
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112
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755
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93
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529
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-226
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FRIEDGES, CHARLES
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ELKO
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JE
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29
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456
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49
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231
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-225
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MAPLE GROVE FARMS
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TYLER
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HO
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109
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578
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120
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358
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-220
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POPP, CHRIS
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PIERZ
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HO
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52
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397
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44
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182
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-215
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WENDT, JAMES
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ST CHARLES
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HO
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77
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517
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86
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303
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-214
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BARTHOLOME-FARM
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GOODHUE
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HO
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213
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537
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272
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323
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-214
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ANDERSON, EUGENE
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WALTHAM
|
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HO
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38
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466
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39
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254
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-212
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ANDERSON, DALE
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WELCH
|
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HO
|
43
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427
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67
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215
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-212
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HELLERMANN, DALE
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MELROSE
|
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HO
|
102
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531
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110
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320
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-211
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WINGERT, JIM & RANDI
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HARMONY
|
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HO
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70
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396
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89
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186
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-210
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LUTZ DAIRY FARM
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HUGO
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HO
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58
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365
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55
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155
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-210
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GERADS, RANDY
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HOLDINGFORD
|
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HO
|
36
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859
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32
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650
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-209
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RYNDA, RICHARD & ANN
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MONTGOMERY
|
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HO
|
104
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516
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71
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309
|
-207
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SCHUTH, JOHN & MARY C
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WABASHA
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HO
|
35
|
332
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37
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125
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-207
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HACKLER, LORAN
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WADENA
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HO
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89
|
420
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90
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215
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-205
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BINDER, HOWARD
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GIBBON
|
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HO
|
84
|
549
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80
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345
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-204
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GORENTZ DAIRY
|
DENT
|
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HO
|
116
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787
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157
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584
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-203
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WAYTASHEK, MIKE &
CATHY
|
LITTLE FALLS
|
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HO
|
53
|
582
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52
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381
|
-201
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VRIEZE, RONALD AND CRAIG
|
RACINE
|
|
HO
|
110
|
576
|
102
|
375
|
-201
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NELSON, CHARLES
|
CUMBERLAND
|
|
HO
|
33
|
327
|
32
|
126
|
-201
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FICK, PAUL
|
PLAINVIEW
|
|
HO
|
58
|
368
|
59
|
168
|
-200
|
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ROLLER, EUGENE JR &
BRENDA
|
HEWITT
|
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HO
|
48
|
479
|
54
|
283
|
-196
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KENNETH ALBERTS
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PINE ISLAND
|
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HO
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603
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406
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625
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212
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-194
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